File information for this data set

Files contain raw demultiplexed ITS sequence data. Below, you will find a break down of the file names & column information for "Metadata.csv"

#File List
Two files for every sample are included. See below for file identification.
  SP##: SPRUCE project, month and date harvested
  FP####: Catalog number, unique identifier
  D#: DNA sample
    2 indicates ITS sample
  R1: Forward read
  R2: Reverse Read
Metadata.xlsx: Sample list and metadata information. See below for column identification.
Metadata.csv: Sample list and metadata information. See below for column identification.
Data.Dictionary.txt: Data set information & metadata column identifications & descriptions.

For information on sample processing, run information, and sample identification, please contact Kirsten Hofmockel (kirsten.hofmockel@pnnl.gov)

Metadata.xlsx Column identification

Catalog.Number
# Units/ Format/ Range: Two letters & 4 numbers
# Description: Sample unique identifier
# Variable Type: Metadata

Sample.ID
# Units/ Format/ Range: Full sample ID
# Description: "8 digit sampling date"_ "Location #" "Topography"_"Depth from hollow surface"_Unique identifier
# Variable Type: Metadata

Date.Sampled
# Units/ Format/ Range: MMDDYYYY
# Description: Date samples were taken
# Variable Type: Metadata

Location
# Units/ Format/ Range: 1-6
# Description: Plot number
# Variable Type: Metadata

Topography
# Units/ Format/ Range: Hummock (Hi) or Hollow (Lo)
# Description: Micro topography location
# Variable Type: Metadata

Depth.Value
# Units/ Format/ Range: cm from hollow surface
# Description: Depth sample was taken
# Variable Type: Metadata

Depth.Text
# Units/ Format/ Range: cm from hollow surface
# Description: Depth sample was taken, POS and NEG indicate positive (Hi only) or negative in relation to hollow surface
# Variable Type: Metadata

Section
# Units/ Format/ Range: Depth
# Description: Depth sections
# Variable Type: Metadata

Set
# Units/ Format/ Range: 1, 2
# Description: Two independent replicate cores within a location
# Variable Type: Metadata

ITS.Sequence.ID
# Units/ Format/ Range: SP####-XX####-X#
# Description: Sample sequence name identification. Identified via Month and Year of sample, catalog number, and sequence type.
# Variable Type: Metadata

Forward Read File
# Units/ Format/ Range: SP####-XX####-X#_R1.fastq
# Description: Forward read file ID.
# Variable Type: Metadata

Reverse Read File
# Units/ Format/ Range: SP####-XX####-X#_R2.fastq
# Description: Reverse read file ID.
# Variable Type: Metadata

ITS.Job.ID
# Units/ Format/ Range: 6 digit date
# Description: YYMMDD date samples were sequenced
# Variable Type: Metadata

ITS.Barcode
# Units/ Format/ Range: 12 letters
# Description: Barcode used to identify samples in demultiplexing step.
# Variable Type: Metadata
